addgene plasmid 153002 (Bio-Rad)
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addgene plasmid 153002
Addgene Plasmid 153002, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/addgene+plasmid+153002/Macro-Prep+25+Q+Support/pm35320713-293-38-66
Average 93 stars, based on 9 article reviews
Addgene Plasmid 153002, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/addgene+plasmid+153002/Macro-Prep+25+Q+Support/pm35320713-293-38-66
Average 93 stars, based on 9 article reviews
addgene plasmid 153002 - by Bioz Stars,
2026-09
93/100 stars
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Plasmid Preparation:Article Title: Functional interactomes of the Ebola virus polymerase identified by proximity proteomics in the context of viral replication. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Benzonase Nuclease, Purity >90% Millipore/Sigma Cat#70746 NuPage Sample reducing agent (103) ThermoFisher Cat#NP0009 NuPage LDS sample buffer (43) (Laemmli) ThermoFisher Cat#NP0008 TRIzolTM Reagent ThermoFisher/Invitrogen Cat# 15596018 DEPC-Treated Water ThermoFisher/Invitrogen Cat#AM9920 Iodoacetamide (IAA) Sigma-Aldrich SKU#I1149-5G Critical commercial assays PierceTM BCA Protein Assay Kit Thermo Scientific Cat#23225 PierceTM Silver Stain for Mass Spectrometry Thermo Scientific Cat#24600 SuperSignalTM West Pico PLUS Chemiluminescent Substrate Thermo Scientific Cat#34578 Luciferase Assay System Promega Cat#E4550 CellTiter-Glo 2.0 Cell Viability Assay Promega Cat#G9241 High-Capacity cDNA Reverse Transcription Kit with RNase Inhibitor ThermoFisher scientific (Applied Biosystems) Cat#4374966 SYBRTM Select Master Mix ThermoFisher scientific (Applied Biosystems) Cat#4472908 Direct-zol RNA Miniprep Plus Zymo research Cat#R2071 ZipTip with 0.2 mL C18 resin Millipore Cat# ZTC18M096 TMT10plexTM Isobaric Label Reagent Set, 1 3 0.8 mg Thermo Scientific Cat# 90110 Deposited data Raw proximity proteomic data of EBOV polymerase interactomes This study MassIVE: PXD031748 Experimental models: Cell lines HEK 293T ATCC Cat#CRL-3216 VERO C1008 [Vero 76, clone E6, Vero E6] ATCC Cat# CRL-1586 Huh7 used in BSL4 A kind gift from Mariano Garcia-Blanco N/A Huh7 used in BSL2 A kind gift from Luke Wiseman N/A Oligonucleotides Primers for RT-qPCRs, see Table S3 This paper N/A siRNAs for knockdowns, see Table S2 This paper N/A AllStars Negative Control siRNA Qiagen Cat#1027281 Oligo dT(20) Primer Thermofisher/invitrogen Cat#18418020 Recombinant DNA Plasmid: pCEZ-NP (Jasenosky et al., 2010) N/A Plasmid: pCEZ-VP35 (Jasenosky et al., 2010) N/A Plasmid: pCDNA5-VP35-HA (Fang et al., 2018) N/A Plasmid: pCAGGS-VP35-HA This study N/A Plasmid: pCAGGS-L A kind gift from Thomas Hoenen N/A Plasmid: pCAGGS-VP35-sNTurboID This study N/A Plasmid: pCEZ-VP30 (Jasenosky et al., 2010) N/A Plasmid: pCEZ-L (Jasenosky et al., 2010) N/A Plasmid: pCEZ-L-sCTurboID This study N/A Plasmid: pHH21-3E5E-Fluc (Jasenosky et al., 2010) N/A Plasmid: pCI-empty This study N/A Plasmid: pCI-MS2V5-eRF3a F76a (Fatscher et al., 2014) Addgene: Cat. #65811; RRID: Addgene_65811 Plasmid: pCI-FLAG-GSPT1 This study N/A (Continued on next page) e2 Cell Reports 38, 110544, March 22, 2022 .. REAGENT or RESOURCE SOURCE IDENTIFIER Plasmid: pCAGGS-eGFP This study N/A Plasmid: pCAGGS-empty This study N/A Plasmid: pLX304 CMV HA-HaloTagFRB-sTurboID (C) (Cho et al., 2020) Addgene: Cat. #153003; RRID: Addgene_153003 Plasmid: pLX304 CMV FKBP-V5sTurboID (N) (Cho et al., 2020) Expressing:Article Title: Functional interactomes of the Ebola virus polymerase identified by proximity proteomics in the context of viral replication. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Benzonase Nuclease, Purity >90% Millipore/Sigma Cat#70746 NuPage Sample reducing agent (103) ThermoFisher Cat#NP0009 NuPage LDS sample buffer (43) (Laemmli) ThermoFisher Cat#NP0008 TRIzolTM Reagent ThermoFisher/Invitrogen Cat# 15596018 DEPC-Treated Water ThermoFisher/Invitrogen Cat#AM9920 Iodoacetamide (IAA) Sigma-Aldrich SKU#I1149-5G Critical commercial assays PierceTM BCA Protein Assay Kit Thermo Scientific Cat#23225 PierceTM Silver Stain for Mass Spectrometry Thermo Scientific Cat#24600 SuperSignalTM West Pico PLUS Chemiluminescent Substrate Thermo Scientific Cat#34578 Luciferase Assay System Promega Cat#E4550 CellTiter-Glo 2.0 Cell Viability Assay Promega Cat#G9241 High-Capacity cDNA Reverse Transcription Kit with RNase Inhibitor ThermoFisher scientific (Applied Biosystems) Cat#4374966 SYBRTM Select Master Mix ThermoFisher scientific (Applied Biosystems) Cat#4472908 Direct-zol RNA Miniprep Plus Zymo research Cat#R2071 ZipTip with 0.2 mL C18 resin Millipore Cat# ZTC18M096 TMT10plexTM Isobaric Label Reagent Set, 1 3 0.8 mg Thermo Scientific Cat# 90110 Deposited data Raw proximity proteomic data of EBOV polymerase interactomes This study MassIVE: PXD031748 Experimental models: Cell lines HEK 293T ATCC Cat#CRL-3216 VERO C1008 [Vero 76, clone E6, Vero E6] ATCC Cat# CRL-1586 Huh7 used in BSL4 A kind gift from Mariano Garcia-Blanco N/A Huh7 used in BSL2 A kind gift from Luke Wiseman N/A Oligonucleotides Primers for RT-qPCRs, see Table S3 This paper N/A siRNAs for knockdowns, see Table S2 This paper N/A AllStars Negative Control siRNA Qiagen Cat#1027281 Oligo dT(20) Primer Thermofisher/invitrogen Cat#18418020 Recombinant DNA Plasmid: pCEZ-NP (Jasenosky et al., 2010) N/A Plasmid: pCEZ-VP35 (Jasenosky et al., 2010) N/A Plasmid: pCDNA5-VP35-HA (Fang et al., 2018) N/A Plasmid: pCAGGS-VP35-HA This study N/A Plasmid: pCAGGS-L A kind gift from Thomas Hoenen N/A Plasmid: pCAGGS-VP35-sNTurboID This study N/A Plasmid: pCEZ-VP30 (Jasenosky et al., 2010) N/A Plasmid: pCEZ-L (Jasenosky et al., 2010) N/A Plasmid: pCEZ-L-sCTurboID This study N/A Plasmid: pHH21-3E5E-Fluc (Jasenosky et al., 2010) N/A Plasmid: pCI-empty This study N/A Plasmid: pCI-MS2V5-eRF3a F76a (Fatscher et al., 2014) Addgene: Cat. #65811; RRID: Addgene_65811 Plasmid: pCI-FLAG-GSPT1 This study N/A (Continued on next page) e2 Cell Reports 38, 110544, March 22, 2022 .. REAGENT or RESOURCE SOURCE IDENTIFIER Plasmid: pCAGGS-eGFP This study N/A Plasmid: pCAGGS-empty This study N/A Plasmid: pLX304 CMV HA-HaloTagFRB-sTurboID (C) (Cho et al., 2020) Addgene: Cat. #153003; RRID: Addgene_153003 Plasmid: pLX304 CMV FKBP-V5sTurboID (N) (Cho et al., 2020) Software:Article Title: Functional interactomes of the Ebola virus polymerase identified by proximity proteomics in the context of viral replication. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Benzonase Nuclease, Purity >90% Millipore/Sigma Cat#70746 NuPage Sample reducing agent (103) ThermoFisher Cat#NP0009 NuPage LDS sample buffer (43) (Laemmli) ThermoFisher Cat#NP0008 TRIzolTM Reagent ThermoFisher/Invitrogen Cat# 15596018 DEPC-Treated Water ThermoFisher/Invitrogen Cat#AM9920 Iodoacetamide (IAA) Sigma-Aldrich SKU#I1149-5G Critical commercial assays PierceTM BCA Protein Assay Kit Thermo Scientific Cat#23225 PierceTM Silver Stain for Mass Spectrometry Thermo Scientific Cat#24600 SuperSignalTM West Pico PLUS Chemiluminescent Substrate Thermo Scientific Cat#34578 Luciferase Assay System Promega Cat#E4550 CellTiter-Glo 2.0 Cell Viability Assay Promega Cat#G9241 High-Capacity cDNA Reverse Transcription Kit with RNase Inhibitor ThermoFisher scientific (Applied Biosystems) Cat#4374966 SYBRTM Select Master Mix ThermoFisher scientific (Applied Biosystems) Cat#4472908 Direct-zol RNA Miniprep Plus Zymo research Cat#R2071 ZipTip with 0.2 mL C18 resin Millipore Cat# ZTC18M096 TMT10plexTM Isobaric Label Reagent Set, 1 3 0.8 mg Thermo Scientific Cat# 90110 Deposited data Raw proximity proteomic data of EBOV polymerase interactomes This study MassIVE: PXD031748 Experimental models: Cell lines HEK 293T ATCC Cat#CRL-3216 VERO C1008 [Vero 76, clone E6, Vero E6] ATCC Cat# CRL-1586 Huh7 used in BSL4 A kind gift from Mariano Garcia-Blanco N/A Huh7 used in BSL2 A kind gift from Luke Wiseman N/A Oligonucleotides Primers for RT-qPCRs, see Table S3 This paper N/A siRNAs for knockdowns, see Table S2 This paper N/A AllStars Negative Control siRNA Qiagen Cat#1027281 Oligo dT(20) Primer Thermofisher/invitrogen Cat#18418020 Recombinant DNA Plasmid: pCEZ-NP (Jasenosky et al., 2010) N/A Plasmid: pCEZ-VP35 (Jasenosky et al., 2010) N/A Plasmid: pCDNA5-VP35-HA (Fang et al., 2018) N/A Plasmid: pCAGGS-VP35-HA This study N/A Plasmid: pCAGGS-L A kind gift from Thomas Hoenen N/A Plasmid: pCAGGS-VP35-sNTurboID This study N/A Plasmid: pCEZ-VP30 (Jasenosky et al., 2010) N/A Plasmid: pCEZ-L (Jasenosky et al., 2010) N/A Plasmid: pCEZ-L-sCTurboID This study N/A Plasmid: pHH21-3E5E-Fluc (Jasenosky et al., 2010) N/A Plasmid: pCI-empty This study N/A Plasmid: pCI-MS2V5-eRF3a F76a (Fatscher et al., 2014) Addgene: Cat. #65811; RRID: Addgene_65811 Plasmid: pCI-FLAG-GSPT1 This study N/A (Continued on next page) e2 Cell Reports 38, 110544, March 22, 2022 .. REAGENT or RESOURCE SOURCE IDENTIFIER Plasmid: pCAGGS-eGFP This study N/A Plasmid: pCAGGS-empty This study N/A Plasmid: pLX304 CMV HA-HaloTagFRB-sTurboID (C) (Cho et al., 2020) Addgene: Cat. #153003; RRID: Addgene_153003 Plasmid: pLX304 CMV FKBP-V5sTurboID (N) (Cho et al., 2020) Cell Analysis:Article Title: Functional interactomes of the Ebola virus polymerase identified by proximity proteomics in the context of viral replication. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Benzonase Nuclease, Purity >90% Millipore/Sigma Cat#70746 NuPage Sample reducing agent (103) ThermoFisher Cat#NP0009 NuPage LDS sample buffer (43) (Laemmli) ThermoFisher Cat#NP0008 TRIzolTM Reagent ThermoFisher/Invitrogen Cat# 15596018 DEPC-Treated Water ThermoFisher/Invitrogen Cat#AM9920 Iodoacetamide (IAA) Sigma-Aldrich SKU#I1149-5G Critical commercial assays PierceTM BCA Protein Assay Kit Thermo Scientific Cat#23225 PierceTM Silver Stain for Mass Spectrometry Thermo Scientific Cat#24600 SuperSignalTM West Pico PLUS Chemiluminescent Substrate Thermo Scientific Cat#34578 Luciferase Assay System Promega Cat#E4550 CellTiter-Glo 2.0 Cell Viability Assay Promega Cat#G9241 High-Capacity cDNA Reverse Transcription Kit with RNase Inhibitor ThermoFisher scientific (Applied Biosystems) Cat#4374966 SYBRTM Select Master Mix ThermoFisher scientific (Applied Biosystems) Cat#4472908 Direct-zol RNA Miniprep Plus Zymo research Cat#R2071 ZipTip with 0.2 mL C18 resin Millipore Cat# ZTC18M096 TMT10plexTM Isobaric Label Reagent Set, 1 3 0.8 mg Thermo Scientific Cat# 90110 Deposited data Raw proximity proteomic data of EBOV polymerase interactomes This study MassIVE: PXD031748 Experimental models: Cell lines HEK 293T ATCC Cat#CRL-3216 VERO C1008 [Vero 76, clone E6, Vero E6] ATCC Cat# CRL-1586 Huh7 used in BSL4 A kind gift from Mariano Garcia-Blanco N/A Huh7 used in BSL2 A kind gift from Luke Wiseman N/A Oligonucleotides Primers for RT-qPCRs, see Table S3 This paper N/A siRNAs for knockdowns, see Table S2 This paper N/A AllStars Negative Control siRNA Qiagen Cat#1027281 Oligo dT(20) Primer Thermofisher/invitrogen Cat#18418020 Recombinant DNA Plasmid: pCEZ-NP (Jasenosky et al., 2010) N/A Plasmid: pCEZ-VP35 (Jasenosky et al., 2010) N/A Plasmid: pCDNA5-VP35-HA (Fang et al., 2018) N/A Plasmid: pCAGGS-VP35-HA This study N/A Plasmid: pCAGGS-L A kind gift from Thomas Hoenen N/A Plasmid: pCAGGS-VP35-sNTurboID This study N/A Plasmid: pCEZ-VP30 (Jasenosky et al., 2010) N/A Plasmid: pCEZ-L (Jasenosky et al., 2010) N/A Plasmid: pCEZ-L-sCTurboID This study N/A Plasmid: pHH21-3E5E-Fluc (Jasenosky et al., 2010) N/A Plasmid: pCI-empty This study N/A Plasmid: pCI-MS2V5-eRF3a F76a (Fatscher et al., 2014) Addgene: Cat. #65811; RRID: Addgene_65811 Plasmid: pCI-FLAG-GSPT1 This study N/A (Continued on next page) e2 Cell Reports 38, 110544, March 22, 2022 .. REAGENT or RESOURCE SOURCE IDENTIFIER Plasmid: pCAGGS-eGFP This study N/A Plasmid: pCAGGS-empty This study N/A Plasmid: pLX304 CMV HA-HaloTagFRB-sTurboID (C) (Cho et al., 2020) Addgene: Cat. #153003; RRID: Addgene_153003 Plasmid: pLX304 CMV FKBP-V5sTurboID (N) (Cho et al., 2020) |
